Crusoe, which builds data centers and runs a GPU cloud for AI training and inference, raised the two largest rounds in this guide: a $1.375 billion Series E in October 2025 at an expected valuation of more than $10 billion, and a $3.9 billion Series F in September 2026 at a $30.9 billion post-money valuation. The company was founded in Colorado and is based in Denver.
The next largest went to True Anomaly, a Centennial company that builds autonomous spacecraft for space defense, Denver kidney care company Strive Health and Boulder drug discovery company Enveda. Most of the other rounds in this guide were between $8 million and $65 million, raised by AI companies working in health care, construction, security, education and enterprise software.
This guide covers notable rounds raised in 2025 and 2026 by AI companies headquartered in Denver, Boulder and the nearby suburbs, the investors who backed them, and the research labs, accelerators and state rules that shape the local market.
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Notable Denver and Boulder AI rounds of 2025
- Crusoe’s $1.375 billion Series E in October 2025 was co-led by Valor Equity Partners and Mubadala Capital.
- Strive Health, whose care teams use machine learning and predictive analytics to slow kidney disease, raised $550 million in September 2025. That was $300 million of Series D equity led by NEA and $250 million of debt led by Hercules Capital.
- True Anomaly raised a $260 million Series C led by Accel in April 2025. Stifel Bank provided part of the money as debt.
- Enveda, which uses AI and machine learning to find drug candidates among natural molecules, added a Sanofi investment in February 2025 that brought its Series C to $150 million. In September it raised a $150 million Series D led by Premji Invest that made it a unicorn.
- Nextworld, a Greenwood Village company whose no-code business software platform lets customers build AI agents for tasks such as invoice processing, raised a $65 million Series F led by the McVaney Investment Partnership in February 2025.
- Swimlane, a Denver security automation company that uses AI agents to triage and investigate alerts, raised $45 million co-led by Energy Impact Partners and Activate Capital in June 2025.
- MagicSchool, which makes AI tools for K-12 teachers and students, raised a $45 million Series B led by Valor Equity Partners in February 2025.
- Frontera Health, an autism care company that uses AI to analyze therapy sessions and cut paperwork for clinicians, raised a $32 million seed round co-led by Lux Capital and Lightspeed Venture Partners in February 2025.
- Multiply Mortgage, which offers lower-rate mortgages as an employee benefit on an AI-based origination platform, raised a $23.5 million Series A led by Kleiner Perkins in March 2025.
- Notable Systems, which automates paperwork and billing for medical equipment suppliers with AI, raised a $12 million Series B co-led by Harbert Growth Partners and Grotech Ventures in April 2025.
Notable Denver and Boulder AI rounds of 2026 so far
Crusoe’s $3.9 billion Series F, announced in September 2026, was co-led by Atreides Management, Mubadala Capital and Valor Equity Partners, with Founders Fund, GIC, NVIDIA, the Qatar Investment Authority, Radical Ventures and TPG also investing. It is left off the chart below so the other rounds stay readable.
- True Anomaly raised $650 million in a Series D co-led by Eclipse and Riot Ventures in April 2026. The round included $50 million of debt from Stifel Bank. True Anomaly builds the Jackal autonomous spacecraft and Mosaic, its software for flight operations and autonomy.
- Enveda raised a $311 million Series E led by Catalio Capital Management in September 2026, bringing its total funding to more than $845 million. The money supports three drug candidates now in clinical trials.
- VieCure, a Greenwood Village company whose software supports treatment decisions at community cancer practices, raised $43 million led by Mitch Rales, a co-founder of Danaher, and Northpond Ventures, in January 2026.
- Sensera Systems, based in Golden, makes solar-powered jobsite cameras and uses AI to analyze the footage for safety, security and progress tracking. It raised a $27 million Series B led by 10 Atlantic Group in February 2026.
- Northslope, which builds AI applications on Palantir’s AIP platform for companies in health care, aerospace and energy, raised a $22 million Series A co-led by Friends & Family Capital and Goldcrest Capital in January 2026.
- LightTable, whose AI reviews construction drawings to catch errors before building starts, raised a $22 million Series A led by Innovation Endeavors in May 2026. It had raised a $6 million seed round led by Primary Venture Partners in 2025.
- Scotch, which sells an AI-based point-of-sale and operating system to liquor stores, raised a $20 million Series A led by VMG Partners in June 2026.
- Circadence, a Boulder company that runs AI-based cyber training ranges for defense and enterprise teams, closed a $16.4 million round led by Seneca Partners in March 2026.
- Great Sky, a Boulder startup building AI computing hardware that combines superconducting circuits with optical links, raised a $14 million seed round led by Bison Ventures, announced in March 2026.
- Luxonis, a Denver company that makes depth cameras with on-device AI for robots, raised a $14 million Series A led by Denali Growth Partners in July 2026, its first institutional round.
- Credible Data, a Boulder company that gives AI agents and analytics tools a company’s agreed business metrics and definitions, raised a $10 million seed round from Gradient, SignalFire, K5 Global and others in July 2026.
- Cloverleaf AI, a Denver company that tracks public meetings and budgets at more than 70,000 government agencies to find sales leads, announced an $8 million Series A led by S3 Ventures in August 2026.
Denver and Boulder AI startups at a glance
The table lists the rounds covered above, newest first.
| Company | City | What it builds | Funding | Date |
|---|---|---|---|---|
| Enveda | Boulder | AI drug discovery from natural molecules | $311M Series E led by Catalio Capital Management | Sep 2026 |
| Crusoe | Denver | AI data centers and GPU cloud | $3.9B Series F, $30.9B post-money valuation | Sep 2026 |
| Cloverleaf AI | Denver | Sales leads from government meetings and budgets | $8M Series A led by S3 Ventures | Aug 2026 |
| Credible Data | Boulder | Business context for AI agents | $10M seed | Jul 2026 |
| Luxonis | Denver | Depth cameras with on-device AI | $14M Series A led by Denali Growth Partners | Jul 2026 |
| Scotch | Denver | Operating system for liquor stores | $20M Series A led by VMG Partners | Jun 2026 |
| LightTable | Denver | AI review of construction drawings | $22M Series A led by Innovation Endeavors | May 2026 |
| True Anomaly | Centennial | Autonomous spacecraft for space defense | $650M Series D, including $50M of debt | Apr 2026 |
| Great Sky | Boulder | AI computing hardware | $14M seed led by Bison Ventures | Mar 2026 |
| Circadence | Boulder | AI cyber training ranges | $16.4M led by Seneca Partners | Mar 2026 |
| Sensera Systems | Golden | Jobsite cameras with AI analysis | $27M Series B led by 10 Atlantic Group | Feb 2026 |
| Northslope | Denver | AI applications on Palantir AIP | $22M Series A | Jan 2026 |
| VieCure | Greenwood Village | Decision support for cancer care | $43M led by Mitch Rales | Jan 2026 |
| Crusoe | Denver | AI data centers and GPU cloud | $1.375B Series E, expected valuation above $10B | Oct 2025 |
| Strive Health | Denver | Kidney care with predictive analytics | $300M Series D equity plus $250M debt | Sep 2025 |
| Enveda | Boulder | AI drug discovery from natural molecules | $150M Series D led by Premji Invest | Sep 2025 |
| Swimlane | Denver | AI security automation | $45M co-led by Energy Impact Partners and Activate Capital | Jun 2025 |
| True Anomaly | Centennial | Autonomous spacecraft for space defense | $260M Series C led by Accel | Apr 2025 |
| Notable Systems | Denver | AI billing for medical equipment suppliers | $12M Series B | Apr 2025 |
| Multiply Mortgage | Denver | Mortgages as an employee benefit | $23.5M Series A led by Kleiner Perkins | Mar 2025 |
| Frontera Health | Denver | AI-supported autism care | $32M seed co-led by Lux Capital and Lightspeed | Feb 2025 |
| MagicSchool | Denver | AI tools for teachers and students | $45M Series B led by Valor Equity Partners | Feb 2025 |
| Nextworld | Greenwood Village | No-code business software with AI agents | $65M Series F | Feb 2025 |
Large Colorado rounds that are not AI companies
Several of the biggest Colorado financings of the period came from aerospace companies that do not build AI products, so they are not in the lists above. Boom Supersonic raised $300 million in December 2025, partly to build a natural gas turbine line that will power AI data centers, with Crusoe as its launch customer. Louisville’s Sierra Space raised a $550 million Series C at an $8 billion valuation in March 2026. Denver’s Voyager Technologies listed on the New York Stock Exchange in June 2025.
Palantir Technologies moved its headquarters from Denver to Aventura, Florida, in February 2026, after six years in Denver.
Who invests in Denver and Boulder AI startups
Most of the large rounds above were led by firms from outside Colorado, including Valor Equity Partners, Mubadala Capital, Atreides Management, Accel, Eclipse, Riot Ventures, Catalio Capital Management, Premji Invest, NEA and Kleiner Perkins. Several of the Colorado firms below invest mainly at pre-seed and seed.
- Foundry Group in Boulder said in January 2024 that its 2022 fund would be its last. It is still investing from that fund.
- Range Ventures is a Denver pre-seed and seed fund. Its focus areas include vertical AI and AI infrastructure and tooling.
- Matchstick Ventures invests at pre-seed and seed in Colorado and Minnesota.
- Access Venture Partners invests at seed and Series A in software, cybersecurity and marketplaces.
- Boulder Ventures invests in information technology and life sciences companies.
- Colorado Impact Fund in Denver invests from late seed to growth stage.
- Stout Street Capital backs pre-seed and seed deep tech companies in fields such as aerospace, biotech, energy and quantum computing.
- Blackhorn Ventures invested in LightTable’s Series A.
Research labs, accelerators and state rules
The federal lab in Golden formerly called the National Renewable Energy Laboratory was renamed the National Laboratory of the Rockies on December 1, 2025. Venture Partners at CU Boulder is the university’s office for licensing research and helping faculty start companies.
Techstars Boulder is back after a pause, now run as a locally owned partnership with Techstars as an investor. Its first new cohort started on September 8, 2026, with Demo Day scheduled for December 7, 2026. The state’s Advanced Industries Accelerator program gives grants of up to $250,000 to companies commercializing technology and up to $150,000 for proof-of-concept work at Colorado universities and federal labs.
In 2024 Colorado became the first US state to pass a law broadly regulating high-risk AI systems, but that law never took effect. On May 14, 2026, Governor Jared Polis signed SB26-189, which repealed it and replaced it with a narrower law on automated decision-making tools used for decisions such as hiring, lending, insurance and health care. The new law is set to take effect on January 1, 2027. xAI’s court challenge to the earlier law is still pending, and a ruling could affect enforcement.
Frequently asked questions
What AI companies are in Denver and Boulder?
AI companies in the area that raised money in 2025 and 2026 include Crusoe, True Anomaly, Enveda, Strive Health, Nextworld, Swimlane, MagicSchool, Frontera Health, Multiply Mortgage, Notable Systems, VieCure, Sensera Systems, Northslope, LightTable, Scotch, Circadence, Great Sky, Luxonis, Credible Data and Cloverleaf AI. Enveda, Circadence, Great Sky and Credible Data are in Boulder. True Anomaly is in Centennial, Sensera Systems is in Golden, and Nextworld and VieCure are in Greenwood Village.
What were the largest Denver and Boulder AI rounds of 2025 and 2026?
Among the rounds in this guide, Crusoe raised the two largest: a $3.9 billion Series F in September 2026 and a $1.375 billion Series E in October 2025. True Anomaly’s $650 million Series D in April 2026 was next, followed by Strive Health’s $550 million in September 2025 ($300 million of equity and $250 million of debt) and Enveda’s $311 million Series E in September 2026.
Which Denver and Boulder AI companies are valued at $1 billion or more?
Crusoe was valued at $30.9 billion after its September 2026 Series F. Enveda became a unicorn with its September 2025 Series D, and it has raised more than $845 million in total. True Anomaly was reported to be valued at $2.2 billion after its April 2026 Series D. Strive Health was reported to be valued at $1.8 billion after its September 2025 round.
Is Palantir still headquartered in Denver?
No. Palantir moved its headquarters from Denver to Aventura, Florida, in February 2026. It had been based in Denver since 2020.
Who invests in Denver and Boulder AI startups?
Colorado investors include Foundry Group, Range Ventures, Matchstick Ventures, Access Venture Partners, Boulder Ventures, Colorado Impact Fund, Stout Street Capital and Blackhorn Ventures. Lead investors on the largest recent rounds include Valor Equity Partners, Mubadala Capital, Atreides Management, Eclipse, Riot Ventures, Accel, Catalio Capital Management and Premji Invest.
For how Denver and Boulder compare with other cities outside Silicon Valley, see Bot Memo’s ranking of tier 2 AI startup hubs, the full AI startup funding by city breakdown, and the guides to AI companies in Austin, Chicago and Atlanta.
About the author
Editorial Staff
The Editorial Staff at Bot Memo is a team of writers, analysts, and AI agents dedicated to mapping the global AI startup ecosystem. Led by Chintan Zalani, the team tracks thousands of funding rounds, classifies companies across verticals, and distills it all into actionable intelligence for investors and founders.
